After sequencing the vectors were transformed into e . coli dh5 a and recombinant fusion protein was expressed via induction of iptg . fusion protein coded by carboxyl terminal gene sequence was purified through glutathione agarose column 經(jīng)sds page分析,在相對分于質(zhì)量( mr )為74000和43000處,各有1條特異的蛋白帶。
The 53kd and 37kd proteins ( named vp53 and vp37 , respectively ) encoded by the 5 ' - terminal genes of rna2 of broad bean wilt virus 2 ( bbwv - 2 ) are c - co - terminally overlapping proteins results from two potential translation sites in rna2 蠶豆萎蔫病毒2 ( bbwv - 2 ) rna2編碼的前體蛋白能切割加工產(chǎn)生一種c ?端重疊的功能未知的蛋白,分子量分別為53kd和37kd ,因而將其命名為vp53 vp37 。
The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure . in recent years , we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion . ccr5 was paid closed attention to since it was cloned in 1996 . the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e . coli and to prepare its specific antibody f ( ab ' ) ; , and its detected method Ccr5具有g(shù)蛋白偶聯(lián)受體家族( g - proteincoupledreceptors , gpcrs )所特有的7個跨膜區(qū)( transmembrinedomains , tm ) ,呈螺旋, tm的氨基酸有很高的保守性,膜外第一襻( extracellularloop1 , ecl1 )和ecl2之間有二硫鍵相連,以維持蛋白質(zhì)二級結(jié)構(gòu)的穩(wěn)定性。
Conclusion : in this paper , we show huccr5 n - terminal gene fragment was cloned successfully and its specific antibody was prpared . by these we not only introduced a simple and quick method to get a specific antibody f ( ab ' ) 2of certain functional domain but also a good idea and technique to study other high similar superfamily members 中有效表達(dá)ccrsn端膜外第一謬基因片段,并制備出抗hllccrsn端的特異性抗體f ( ah ’ ) 。 。本方法為一簡捷快速的特定功能結(jié)構(gòu)域抗體廠( ah ’ ) 。